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Standard soil with specific characteristics is amended with a suitable organic substrate and mixed directly, or by an appropriate carrier, with different concentrations of the test substance in order to evaluate the effects on microbial nitrate formation rate. The deviation (%) in nitrate formation rate respect to the untreated soil control is assessed at defined intervals until 28 days after the treatment or for a maximum of 100 days until a difference ≤ 25 % is obtained.
Agricultural loamy sand soil.
A toxic reference substance is tested once a year to check the sensitivity of the soil microoorganisms.
For agrochemicals products 2 concentrations and 1 untreated control are tested
(3 replicates/concentration and for the control).
For substances other than agrochemicals, a dose-response test for ECx evaluation is carried out. It includes at least 5 concentrations and 1 untreated control (3 replicates/concentration and for the control).
% of deviation in nitrate formation rate of treated soils respect to the untreated soil, at the end of the test.
Study includes GLP management and reporting.
REFERENCES AND GUIDELINES
OECD Guideline fo Testing of Chemicals, No. 216 (21st January 2000) - Soil microorganisms: Nitrogen transformation test.